T7 RNA polymerase is a 99kDa DNA dependent RNA polymerase which initiates RNA synthesis after encountering a T7 phage promoter sequence on the DNA template. This enzyme is essential for in-vitro transcription of a template DNA and hence, is indispensable for the manufacture of mRNA therapeutics and vaccines. At Loka Biosciences, we offer high quality T7 RNA polymerase suitable for all research/pre-clinical/clinical applications.

Advantages for choosing Loka’s T7 RNA Polymerase:

 Manufactured under GMP rules and regulations

 Completely animal origin free manufacturing process

 Free of Endotoxins and antibiotics

 Supplied with adequate documentation validating the quality

Applications

T7 RNA Polymerase transcribes RNA using a DNA/plasmid template carrying the T7 promoter sequence efficiently incorporating ribonucleoside triphosphates (NTPs) into mRNA transcripts.

T7 RNA polymerase is routinely used for research and clinical applications such as:

 Preparation of radio labeled RNA probes

 Non-isotopic RNA labeling

 Manufacture of mRNA based vaccines

 Manufacture of mRNA based therapeutics

 Preparation of guide RNA for gene targeting

 mRNA for micro injection and in-vitro translation

 RNA structure, processing and catalysis studies

 RNA amplification

 Anti-sense RNA for gene expression experiment

RNAse Inhibitor from LOKA

RNase Inhibitor, Murine is a 50 kDa recombinant protein of murine origin. The inhibitor specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity.

In addition RNase Inhibitor is being usedused with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).

Rnase Inhibiotor Isolated from E.coli carrying a plasmid which contains the Rnase Inhibiotor gene. 

DNAse (RNAse Free) From LOKA

DNase I, (RNase-free) is an endonuclease that non-specifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids.

DNAse I Isolated from E.coli carrying a plasmid which contains the Dnase I gene. 

It is used in the DNA removal from IVT mRNA preparations, Recombinant therapeutics and Vaccines bulk preparations